(a known antigen→ patient serum which contains antigen specific antibody→)
☆Anti-human immunoglobulin antibody coupled to a substrate-modifying enzyme (eg, peroxidase) is then added. This antibody-enzyme conjugate binds to antigen-bound antibodies in the wells. The plate is again washed.
(→subtrate is added to elicit a detectable signal such as color change(proportional to the amount of antibody present in the patient's serum))
immunology
UWorld SIM1
Optimized studying based on the forgetting curve vastly improves memorization. - What is reminDO?
Memorize
